Capture clear, low-background images every time without adjusting camera settings. See More with Additional Channels and Clearer Images: Document DNA gels, image ECL blots, and detect multiple targets on the same blot with two channel near-infrared fluorescence.Do More by Going Digital: Get consistent, reproducible digital images, without the hassles and unpredictability of film – so you can analyze your data right away and plan your next experiment.The Odyssey Fc System allows your lab to combine the proven advantages of infrared fluorescence detection with simple, streamlined qualitative chemiluminescence detection on one instrument. The Odyssey Fc System is the only imaging system that offers superior chemiluminescent detection and the benefits of quantitative infrared Western blot detection as well as the ability to document DNA gels using the 600nm channel. LC-MS innovation: Improve analytical accuracy Which microbioreactor modules are best for you It is the best one and most economical one available at the present time.Which solid support is right for your oligo synthesis? The quote was not significantly reduced either. Disappointed that the software is expensive. I was a bit disappointed that no one came to train us and email replies of reps once we bought it were abrupt. I wish it also took pictures of the blot for the molecular weight standard marking but we have the option of using the glow stick for that. We used Millipore substrate and it worked really well with it. We were told by another lab that we have to buy ECL substrates from LI-COR for it to work, but that is not the case. Once the machine is done scanning, you are again given a chance to choose the best exposures for your use. Once you standardize the intensity using the provided target blot, you can either choose a standard sensitivity (6 mins acquisition time) or high sensitivity (12 mins). " C-DiGit™ Blot Scanner for Chemiluminescent Western Blots by Li-Cor is a wonderful product. Review date: | C-DiGit® Blot Scanner for Chemiluminescent Western Blots And at the end of the day, you'll get more accurate quantitative data than you would with film." I've successfully blotted HIF-1alpha from a whole cell lysate on this machine, which is notoriously difficult to detect. Once you know the limitations and tools available, there shouldn't be very many blots you can't get to work on this. They have resources, but they're too minimalistic. It would be wise for Licor to be more forward about this and help users view their fussy blots on the machine from the get-go. That being said, this problem is easily overcome by using stronger substrates (which can also be diluted with water to your likeness!) and/or by improving their western blot technique. An 11 minute exposure on the machine, even when a blot is directly placed on the machine with no plastic wrap, is indisputably not equivalent to an 11 minute exposure on film. We had a lot of people try the machine once and swear to never use it again. So why did I rate it 3 stars in that category? Well, people who routinely do very long exposures on film are going to wonder why it's not working out for them, and I don't think Licor has been forward enough about the sensitivity, which, in my view, is 40-60% less sensitive than film. Anyway, here's a candid view from someone that has regularly used this for two years.įor one, the machine is very easy to use. " A lot of the reviews on this site seem like they're written by a marketing company. Which solid support is right for your oligo synthesis?
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